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KMID : 0351619670080010055
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Kyungpook Medical Journal
1967 Volume.8 No. 1 p.55 ~ p.92
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Effects of Cortisone on the Growth of Various Organs in Chick Embryos
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Abstract
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This study is to observe the effects of cortisone on the weight gains of body and various organs of chick embryo, out of which growth and relative growth formula has been derived. On the 8th and the 12th days of incubation, 0.5mg and 2.0mg of cortisone were injected into the chorioallantoic membrane of each incubated egg of two groups. Between the 12th and 18th of Incubation days, a daily total of 10 treated eggs was broken for weighing the body weights of chicks embryos and six organs-brain, eye ball, heart, lung, liver, and kidney. The effects of doses and difference of injection times on growth were analyzed by the Repeated Two Way Layout Method, and the determination of the significance in the levels of difference was made by the Turkey´s Method. The formulas applied to analyzing the changes in growth are : y=a+bt+ct², coefficient of relative growth (α) by y=bxα. y=growth quantity of organs ; t= specific date of incubation period, x=bady weight ; a, b, c= constants.
The results are summarized as follows :
(1) When injected with cortisone acetate, the chick embryos were observed with remarkable growth inhibition(p<0.01).
(2) The growth inhibition appeared to be much stronger with the group of 2.0mg dosage than that of 0.5mg dosage. No noticeable differences were observed on the liver of the 8th day group, and on the kidneys of the 12th day group.
(3) Generally, the growth inhibition was found strenger on the 8th day group than on the 12th(P<0.01), but in brain and eye balls, no inhibitory difference depending on the injection days was observed. Lungs were unique in that the growth inhibition appeared in the 12th day group (p<0.01).
(4) Growth rate : In the 8th day group, the growth rate was observed to be increased in body weight, lungs, liver, and kidneys throughout the two injected groups- 0.5mg and 2.0mg : brain and heart, however, showed the increase only in 2.0mg injected group. The growth rate was obsereved decreased in eye balls of two injected groups ; the decrease of brain and heart were in 0.5mg injected groups.
In the 12th day group, the increase in growth rate was shown in the followings : body weight, eye balls and lungs of 0.5mg injected group, body weight, eye balls and kidneys of 2.0mg injected group, the other organs except the increased ones showed decrease in all injected groups.
(5) Specific Growth Rate :
(a) In the 8th day group : 1) increase was obrerved only in lungs, 2) decreaee was in all organs except lungs.
(b) In the 12th day group : 1) increase eye balls of 2.0mg injected group ; kidneys of 2. 0mg group. 2) decrease was in the other organs except the above mentioned.
(6) The Growth Gradient : The growth centers appeared as follows :
(a) In the 8th day group : lungs of all two injected groups, on the 18th day of incubation ; liver of 2.0mg injected group, on the 15th ; kidneys of 0.5mg injected group, 17th day of incubation ; the others all appeared on the 12th day.
(b) In the 12th day group : brain, heart and liver of 0.5mg injected group had the growth centers on the 12th incubation day, and the others had them on the 18th incubation day.
(7) The Coefficient of Relative Growth : The coefficient of relative growth was observed as follows :
(a) In the 8th day group : the liver of two injected group, and kidneys of 0.5mg injected group were of positive allometry : the others showed negative.
(b) In the 12th day group : liver of two injected groups and heart and kidneys of 2.0mg injected group were positive, while the others were negative.
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